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Z scores were plotted according to Z = (x - μ)/σ, where x is the log 2 transformed gene expression measurement and μ and σ are the mean and standard deviations of expression of the gene. Red and blue colors indicate high and low relative expression levels after homogenization, respectively. The RPKM value for each gene and each stage was provided in the figure. In the gonad, two ( hsd3b1 and hsd17b14 ) and eight ( hsd3b7 , hsd17b1 , hsd17b7l , hsd17b10 , hsd17b12a , hsd17b12b , hsdl1 , and hsdl2 ) β -HSD genes were up- and downregulated during sex reversal, respectively. Hsd3b1 catalyzes the second step of steroidogenesis and is required for the synthesis of all steroids including 11-KT (Hsu et al., 2009). In human testis specimens, HSD17B14 protein has shown immunoreactivity in most of the seminiferous epithelium as well as in peritubular areas harboring Leydig cells (Sivik et al., 2012).
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