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Genome-Wide Identification of β -hsd Genes. To identify candidate β -hsd genes in these thirteen species, we collected the gene and protein names of the β -hsd gene family from the literature (Payne et al., 1997; Dai et al., 2003; Mindnich et al., 2004; Seckl, 2004; Hoffmann and Maser, 2007; Meier et al., 2009; Mindnich and Adamski, 2009; Sreenivasulu and Senthilkumaran, 2009; Rotinen et al., 2010; Kisiela et al., 2012; Saloniemi et al., 2012; Tokarz et al., 2012; Lin et al., 2013; He et al., 2018). Secondly, the amino acid sequences of human and zebrafish β -hsd genes were collected from Ensembl and GenBank by searching for their gene and protein names. Next, specific hidden Markov models (HMMs) of human and zebrafish β-HSDs were developed by hmmbuild command in HMMER (HMMER3.1B2 13 ) and then used as the query sequences to search the protein databases of all the studied species with hmmsearch command in HMMER ( E -value = 0.01). Chain – several single bets on irrelevant favoritos bbb22 outcomes. Therefore, the amino acid sequence of human HSD17B5 was used as query sequence to search the protein databases of other species by local BLASTP with a cut off E -value of 1e-5 (BLAST 2.2.26 release from NCBI by anonymous FTP 14 ) (Altschul et al., 1997). All the suspected sequences were aligned with the NCBI non-redundant protein sequence database by online BLASTP program to obtain reliable sequences of β -hsd genes.

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Get The Poker Lab Recommended Preflop Ranges. 4-Betting When Facing a 3-Bet In Position. 4-betting in position puts the 3-bettor in a very tricky spot and increases the likelihood of them making a mistake. These premium pocket pairs play best when there is a small stack-to-pot-ratio and isolated opponents, and 4-betting them will cultivate both of these conditions. By 4-betting this AK, we accomplish one of three things: Consider these alongside our positional advantage and it becomes clear why AK is a favorable hand to 4-bet with. Mixing in more thin value-bets like these allow us to bluff more often as well, which punishes the 3-bettor for their over-aggressiveness. Now, we have to balance our 4-betting range by adding in some bluffs. Bet streaming. On the other hand we have the Fold to 3-Bet stat, which comes in handy when we have to decide whether to re-raise an opponent – especially preflop. Full ring table, we have AQ offsuit in MP and raise 3xBB. A player in LP re-raises us 9xBB. Everyone else folds and the action is back to us. What should we do? Let’s look at the stats.
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